Home > Sandwich ELISA Kits > Human WD Repeat And SOCS Box Containing Protein 1 (WSB1) ELISA Kit

Product Name:

Human WD Repeat And SOCS Box Containing Protein 1 (WSB1) ELISA Kit

Cat.#:

JL17382

Regulatory Status:

RUO

Alternative:

SWIP1; WSB-1; SOCS box-containing WD protein SWiP-1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.153ng/mL

Specificity:

This kit recognizes Human WSB1 in samples. No significant cross-reactivity or interference between Human WSB1 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| WSB1

WD repeat and SOCS box-containing protein 1 is a protein that is encoded by the WSB1 gene.This gene encodes a member of the WD-protein subfamily. This protein shares a high sequence identity to mouse and chick proteins. It contains several WD-repeats spanning most of the protein and an SOCS box in the C-terminus. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.66	1.97	1.36	0.83	0.51	0.32	0.2	0.08
Corrected OD	2.58	1.89	1.28	0.75	0.43	0.24	0.12	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human WSB1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	93-105	102
Cell Culture Supernates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Human WSB1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	85-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	109